Urologic Oncology: Seminars and Original Investigations
Volume 30, Issue 2 , Pages 177-181 , March 2012

Hypermethylation of E-cadherin, p16, p14, and RASSF1A genes in pathologically normal urothelium predict bladder recurrence of bladder cancer after transurethral resection

  • Hui-Hui Lin, B.S.

      Affiliations

    • Department of Microbiology, Kaohsiung Medical University, Kaohsiung, Taiwan
    • Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
    • ,
  • Hung-Lung Ke, M.D., M.S.

      Affiliations

    • Department of Urology, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan
    • Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
    • ,
  • Wen-Jeng Wu, M.D., Ph.D.

      Affiliations

    • Department of Urology, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan
    • Department of Urology, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
    • ,
  • Ying-Huei Lee, M.D., Ph.D.

      Affiliations

    • Department of Urology, Chi Mei Medical Center, Tainan, Taiwan
    • These authors contributed equally to this work.
    • ,
  • Lin-Li Chang, Ph.D.

      Affiliations

    • Department of Microbiology, Kaohsiung Medical University, Kaohsiung, Taiwan
    • Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
    • Corresponding Author InformationCorresponding author. Tel.: +886-7-3121101, ext: 2150; fax: +886-7-3211033
    • These authors contributed equally to this work.

Received 8 October 2009 ,Revised 8 January 2010 ,Accepted 9 January 2010.

References 

  1. Annual Report of Cancer Registry System . Bureau of Health Promotion, Department of Health, Republic of China . 2003;
  2. Rubben H , Lutzeyer W , Fischer N , et al.  Natural history and treatment of low and high risk superficial bladder tumors . J Urol . 1988;139:283–285
  3. Kakizoe T . Development and progression of urothelial carcinoma . Cancer Sci . 2006;97:821–828
  4. Habuchi T , Takahashi R , Yamada H , et al.  Metachronous multifocal development of urothelial cancers by intraluminal seeding . Lancet . 1993;342:1087–1088
  5. Esteller M , Corn PG , Baylin SB , et al.  A gene hypermethylation profile of human cancer . Cancer Res . 2001;61:3225–3229
  6. Catto JW , Azzouzi AR , Rehman I , et al.  Promoter hypermethylation is associated with tumor location, stage, and subsequent progression in transitional cell carcinoma . J Clin Oncol . 2005;23:2903–2910
  7. Muto S , Horie S , Takahashi S , et al.  Genetic and epigenetic alterations in normal bladder epithelium in patients with metachronous bladder cancer . Cancer Res . 2000;60:4021–4025
  8. Chang LL , Yeh WT , Yang SY , et al.  Genetic alterations of p16INK4a and p14ARF genes in human bladder cancer . J Urol . 2003;170:595–600
  9. Maruyama R , Toyooka S , Toyooka KO , et al.  Aberrant promoter methylation profile of bladder cancer and its relationship to clinicopathologic features . Cancer Res . 2001;61:8659–8663
  10. Lee MG , Kim HY , Byun DS , et al.  Frequent epigenetic inactivation of RASSF1A in human bladder carcinoma . Cancer Res . 2001;61:6688–6692
  11. Dominguez G , Carballido J , Silva J , et al.  p14ARF promoter hypermethylation in plasma DNA as an indicator of disease recurrence in bladder cancer patients . Clin Cancer Res . 2002;8:980–985
  12. Epstein JI , Amin MB , Reuter VR , et al.  The World Health Organization/International Society of Urological Pathology consensus classification of urothelial (transitional cell) neoplasms of the urinary bladder. Bladder Consensus Conference Committee . Am J Surg Pathol . 1998;22:1435–1448
  13. Greene FL , Page DL , Fleming ID , et al.  AJCC Cancer Staging Manual . New York: Springer-Verlag; 2002;
  14. Herman JG , Graff JR , Myohanen S , et al.  Methylation-specific PCR: A novel PCR assay for methylation status of CpG islands . Proc Natl Acad Sci U S A . 1996;93:9821–9826
  15. Vucic EA , Brown CJ , Lam WL . Epigenetics of cancer progression . Pharmacogenomics . 2008;9:215–234
  16. Bornman DM , Mathew S , Alsruhe J , et al.  Methylation of the E-cadherin gene in bladder neoplasia and in normal urothelial epithelium from elderly individuals . Am J Pathol . 2001;159:831–835
  17. Dhawan D , Hamdy FC , Rehman I , et al.  Evidence for the early onset of aberrant promoter methylation in urothelial carcinoma . J Pathol . 2006;209:336–343
  18. Chan MW , Chan LW , Tang NL , et al.  Hypermethylation of multiple genes in tumor tissues and voided urine in urinary bladder cancer patients . Clin Cancer Res . 2002;8:464–470
  19. Takeshima M , Saitoh M , Kusano K , et al.  High frequency of hypermethylation of p14, p15, and p16 in oral precancerous lesions associated with betel-quid chewing in Sri Lanka . J Oral Pathol Med . 2008;37:475–479
  20. Tada Y , Wada M , Taguchi K , et al.  The association of death-associated protein kinase hypermethylation with early recurrence in superficial bladder cancers . Cancer Res . 2002;62:4048–4053

☆ This study was sponsored by the Department of Urology, Chi Mei Medical Center, Tainan, Taiwan, and the Department of Urology, Chung-Ho Memorial Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan (95CM-KMU-08).

PII: S1078-1439(10)00005-0

doi: 10.1016/j.urolonc.2010.01.002

Urologic Oncology: Seminars and Original Investigations
Volume 30, Issue 2 , Pages 177-181 , March 2012

Article Tools

* Image Usage & Resolution